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Review
. 2012;29(5):455-62.
doi: 10.3109/02652048.2012.658443. Epub 2012 Feb 21.

Microencapsulated drug delivery: a new approach to pro-inflammatory cytokine inhibition

Carl W Oettinger  1 , Martin J D'Souza
Affiliations
Free PMC article
Review

Microencapsulated drug delivery: a new approach to pro-inflammatory cytokine inhibition

Carl W Oettinger et al. J Microencapsul. 2012.
Free PMC article

Abstract

Context: This article reviews the use of albumin microcapsules 3-4 µm in size containing cytokine inhibiting drugs which include neutralizing antibodies to TNF and IL1, CNI-1493, antisense oligonucleotides to TNF and NF-kappaB, and the antioxidant catalase.

Objective: Describe the effects, cellular uptake and distribution of microencapsulated drugs and the effect in both a peritonitis model of infection and a model of adjuvant-induced arthritis.

Methods: The studies performed by our group are reviewed, the only such studies available.

Results: Microencapsulation of these compounds produced high intracellular drug concentrations due to rapid uptake by phagocytic cells, including endothelial cells, without toxicity. All compounds produced excellent inhibition of TNF and IL1 resulting in improved animal survival in a peritonitis model of septic shock and inflammation in an arthritis model.

Conclusion: Albumin microencapsulated pro-inflammatory cytokine inhibiting compounds are superior to equivalent concentration of these compounds administered in solution form.

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Figures

Figure 1
Figure 1
Scanning electron micrograph of albumin microspheres (magnification ×5500), size 1–7 μm.
Figure 2
Figure 2
Serial photographs of fluorescein-labelled albumin microspheres incubated with macrophages. Progressive phagocytosis of the microspheres is seen.
Figure 3
Figure 3
Endothelial cells were incubated with endotoxin. Fluorescein-labelled albumin microspheres were added and the percentage of cells containing microspheres was determined after 0.5, 1.0, 4.0 and 24 h. *p < 5 0.05 compared to the control group. Increased microsphere uptake by phagocytic cells was noted after endotoxin exposure.
Figure 4
Figure 4
Scanning electron micrograph of albumin microspheres (magnification ×5500), size 1–7 μm.
Figure 5
Figure 5
Development of arthritis and tissue swelling in rats after subcutaneous injection with M. butyricam.
See this image and copyright information in PMC

References

    1. Akhavein N, Oettinger C, Gayakawad S, Addo R, Bejugam N, Bauer J, Pollock S, D'Souza M. Treatment of adjuvant arthritis using microencapsulated antisense to NF-κB oligonucleotides. J Microencapsulation. 2008;18:1–12. - PubMed
    1. Andreakos ET, Feldman M. Autoimmunity and cytokines: Pathogenesis and therapy. In: Thomsom AW, Lotze MT, editors. The cytokine handbook. 4th ed. II. San Diego, CA: Academic Press; 2003. pp. 1189–211.
    1. Bagby G, Plessala K, Wilson L, Thompson J, Nelson S. Divergent efficacy of antibody to tumor necrosis factor-α in intravascular and peritonitis models of sepsis. J Infect Dis. 1991;163:83–8. - PubMed
    1. Bender A, Schafer V, Steffan AM, Royer C, Kreuter J, Rubsamen-Waigmann H, von Briesen H. Inhibition of HIV in-vitro by antiviral drug-targeting using nanoparticles. Res Virol. 1994;145:215–20. - PubMed
    1. Bianchi M, Bloom O, Raabe T, Cohen PS, Chesney J, Sherry B, Schmidtmayerova H, Calandra T, Zhang X, Bukrinsky M. Suppression of proinflammatory cytokines in monocytes by a tetravalent guanylhydrazone. J Exp Med. 1996;183:927–36. - PMC - PubMed

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