Identification of genes potentially related to biomineralization and immunity by transcriptome analysis of pearl sac in pearl oyster Pinctada martensii

Abstract

Pearl oyster Pinctada martensii is cultured for production of pearl in China. It needs to implant a mantle graft cut from a donor oyster and a seed nucleus into the gonad of the host oyster to produce a pearl. Pearl sac surrounding the nucleus is formed by the proliferation of the implanted mantle graft from the outer mantle epithelial cells in the host oyster. The pearl sac is responsible for production of a cultured pearl. A comprehensive transcriptome analysis on pearl sac will help to understand the mechanism on pearl formation and immune response of host oyster after nucleus implantation. In the present study, 39,400,004 reads were produced from the pearl sac using RNA-sequence technology and then assembled into 102,762 unigenes. More than 22.4% of these unigenes were possibly involved in approximately 219 known signaling pathways. A total of 37,188 unigenes were annotated based on sequences similarities with known proteins. Fifty-one biomineralization-related unigenes and 268 immune-related unigenes were not previously detected in P. martensii. The un-annotated unigenes may be some genes specifically existed in P. martensii. These annotated or un-annotated unigenes in the present studies were valuable for the future investigation on molecular mechanism of pearl formation and immune response of the species.