Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1990 Oct 25;18(20):6061-8.
doi: 10.1093/nar/18.20.6061.

DNA sequences required for yeast actin gene transcription do not include conserved CCAAT motifs

J M Munholland  1 , J K Kelly, A G Wildeman
Affiliations
Free PMC article
Comparative Study

DNA sequences required for yeast actin gene transcription do not include conserved CCAAT motifs

J M Munholland et al. Nucleic Acids Res. .
Free PMC article

Abstract

Sequences required for Saccharomyces cerevisiae actin gene transcription were mapped and compared to the regulatory region of the actin gene from a thermophilic fungus, Thermomyces lanuginosus. Two CCAAT motifs conserved in position in these two species could be mutated without affecting promoter activity, regardless of whether the yeast were grown in fermentable or non-fermentable carbon sources. Two TATA-like sequences and an upstream activation sequence (UAS) composed of multiple elements were identified. The contribution of sequence motifs within these elements to UAS activity varied depending on the carbon source. The Thermomyces gene contains sequences highly homologous to this UAS, but in the opposite orientation.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 - PubMed
    1. Genes Dev. 1990 Apr;4(4):503-14 - PubMed
    1. Science. 1982 Jul 23;217(4557):371-3 - PubMed
    1. Nature. 1983 Dec 15-21;306(5944):704-7 - PubMed
    1. Cell. 1984 Feb;36(2):503-11 - PubMed

Publication types