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. 1987 Oct;1(1):7-12.
doi: 10.1007/BF00351115.

Cytological analysis and sorting of a human supernumerary minichromosome

L Ferretti  1 , E Raimondi, L Davis, M Romagnoni, L De Carli, V Sgaramella, B D Young
Affiliations

Cytological analysis and sorting of a human supernumerary minichromosome

L Ferretti et al. Cytotechnology. 1987 Oct.

Abstract

In a newborn female, an abnormal karyotype, 46,XX/47,XX,+mar/47,XX,+9, was found associated with several malformations. The marker chromosome was present in 70% of peripheral blood lymphocytes, and its size appeared to be less than half of the smallest chromosomes. Several differential staining methods provided no indication as to its origin.Chromosomes isolated from EBV-immortalized lymphocytes of the patient were fractionated on a FACS-440. The marker was resolved as a sharp peak in the region close to the chromosomal debris: its DNA content seemed to be close to 40% of chromosomes 21 and 22.About 580000 minichromosomes were sorted. In order to optimize cloning conditions, a pilot cloning experiment was performed on a pool of sorted chromosomes 9, 10, 11 and 12.

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References

    1. Hum Genet. 1985;69(3):268-71 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 May;77(5):2829-33 - PubMed
    1. Science. 1976 Mar 26;191(4233):1268-70 - PubMed
    1. Chromosoma. 1981;83(3):431-9 - PubMed
    1. Nature. 1981 Oct 1;293(5831):374-6 - PubMed