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. 1987 Oct;1(1):25-31.
doi: 10.1007/BF00351118.

Localization of amplified CAD genes on rearranged chromosomes of Chinese hamster cells

L Ottaggio  1 , C Agnese, S Bonatti, P Cavolina, A Di Leonardo, M Miele, A Abbondandolo
Affiliations

Localization of amplified CAD genes on rearranged chromosomes of Chinese hamster cells

L Ottaggio et al. Cytotechnology. 1987 Oct.

Abstract

Chinese hamster cell lines carrying an amplified CAD region were selected from V79,B7 cells by their resistance to N-phosphonacetyl-L-aspartate (PALA). In one of the selected cell lines, SP PALA (inf1) (supR) L, an acrocentric chromosome with abnormally elongated q arms was identified as a marker for the PALA-resistant phenotype. The marker chromosome carried a homogeneously staining region close to a telomeric nucleolar organizer region. In the same region, localization of amplified CAD sequences was demonstrated by in situ hybridization. The marker chromosome was found to undergo extensive rearrangements. In particular, dicentric chromosomes, occurring with an unusually high incidence, were found to originate from end-fusion of two homologous marker chromosomes.

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References

    1. J Biol Chem. 1974 Nov 10;249(21):6945-50 - PubMed
    1. Mol Cell Biol. 1983 Apr;3(4):699-711 - PubMed
    1. Mol Cell Biol. 1983 Nov;3(11):2066-75 - PubMed
    1. Mol Cell Biol. 1982 Mar;2(3):302-7 - PubMed
    1. J Biol Chem. 1977 Sep 25;252(18):6379-85 - PubMed

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