Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Jan;23(1-3):61-75.
doi: 10.1023/B:CYTO.0000010399.23373.65.

Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers

D E Martens  1 , E A Nollen, M Hardeveld, C A Velden-de Groot, C D Gooijer, E C Beuvery, J Tramper
Affiliations

Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers

D E Martens et al. Cytotechnology. 1997 Jan.

Abstract

The death rate of Vero cells grown on Cytodex-3 microcarrierswas studied as a function of the gas flow rate in a smallair-lift loop reactor. The death rate may be described byfirst-order death-rate kinetics. The first-order death-rateconstant as calculated from the decrease in viable cells, theincrease in dead cells and the increase in LDH activity islinear proportional to the gas flow rate, with a specifichypothetical killing volume in which all cells are killed ofabout 2.10(-3)m(3) liquid per m(3) of air bubbles.In addition, an experiment was conducted in the sameair-lift reactor with Vero cells grown inside porous Asahimicrocarriers. The specific hypothetical killing volumecalculated from this experiment has a value of 3.10(-4)m(3) liquid per m(3) of air bubbles, which shows thatthe porous microcarriers were at least in part able to protectthe cells against the detrimental hydrodynamic forcesgenerated by the bubbles.

PubMed Disclaimer

References

    1. Bavarian F, Fran LS, Chalmers J. Microscopic visualization of insect cell bubble interactions. I: Rising bubbles, air-medium interface, and the foam layer. Biotechnol Prog. 1991;7:140–150. - PubMed
    1. Beverloo WA, Tramper J. Intensity of microcarrier collisions in turbulent flow. Bioproc Eng. 1994;11:177–184.
    1. Bugarski B, King GA, Daugulis AJ, Goosen MFA. Performance of an external air-lift bioreactor for the production of monoclonal antibodies by immobilized hybridoma cells. Applied Microbiol Biotechnol. 1989;30:264–269.
    1. Cahn F. Biomaterials aspects of porous microcarriers for animal cell culture. Tibtech. 1990;8:131–136. - PubMed
    1. Chalmers JJ, Bavarian F. Microscopic visualization of insect cell-bubble interactions. II: The bubble film and bubble rupture. Biotechnol Prog. 1991;70:151–158. - PubMed