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. 1989 Feb;2(1):5-8.
doi: 10.1007/BF00365409.

High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium

M Tokashiki  1 , T Arai
Affiliations

High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium

M Tokashiki et al. Cytotechnology. 1989 Feb.

Abstract

Mouse-human hybridoma X87X cells were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0×10(7) cells/ml, when the specific perfusion rate was set to 2.3 vol day(-1), and monoclonal antibody was continuously produced. These results were almost the same as those in the perfusion culture vessel with one settling zone and revealed that the process with a plurality of settling zones is a promising one for scale-up of a gravitation type of perfusion culture vessel.

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References

    1. Science. 1972 Oct 6;178(4056):65-6 - PubMed
    1. Science. 1969 May 2;164(3879):555-7 - PubMed

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