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. 1997 Nov;25(1-3):25-33.
doi: 10.1023/A:1007935026770.

Reinforcing apoptosis-resistance of COS and myeloma cells by transfecting with bcl-2 gene

T Fujita, S Terada, K Fukuoka, A Kitayama, H Ueda, E Suzuki

Reinforcing apoptosis-resistance of COS and myeloma cells by transfecting with bcl-2 gene

T Fujita et al. Cytotechnology. 1997 Nov.

Abstract

COS, myeloma and HeLa cells, which are commonly used for protein production by cell culture, were transfected with human bcl-2 gene encoded on the shuttle vector BCMGS. Expression of human bcl-2 improved survival of cells remarkably, mildly, or negligibly for COS, myeloma, and HeLa, respectively. Four clones were obtained from the human bcl-2 expressing cell population of COS cells. They expressed human bcl-2 almost at the same level. The viable cell numbers were 6, 2.5, 2.5, and 0.8 times as many for the clones #8, #5, #6, and #7, respectively, as for the control COS cells, when they were cultured at low (0.2%) serum concentration for 9 days. The bcl-2 overexpressing COS cells showed morphology different from that of the control COS cells in serum limited condition. When transfected with mouse lambda protein gene carried by an SV40-derived vector, clone #8 of the bcl-2 transfected COS cells continued the transient expression of lambda protein longer than the control COS cells.

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