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. 1999 Mar;29(2):103-13.
doi: 10.1023/A:1008092620572.

DNA content analysis of insect cell lines by flow cytometry

X Léry  1 , G Charpentier, S Belloncik
Affiliations

DNA content analysis of insect cell lines by flow cytometry

X Léry et al. Cytotechnology. 1999 Mar.

Abstract

The DNA content of insect cell lines (6 lepidoptera, 1 coleoptera and 1 diptera) was determined by flow cytometry. The DNA profiles of the 8 cell lines tested were different. They were characterized by the presence of several peaks (2 to 7) corresponding to different ploidy levels, by differences in the fluorescence intensity of each peak and by the proportion of cells in each peak. Two cell lines (Cf124 and BmN) were constituted of 2 distinct populations of cells. The DNA profiles of the cell lines were stable among the passages and during the length of time culture. This technique was demonstrated to be useful for the detection of mixed cell lines and nucleopolyhedrovirus cell infection, using Autographa californica MNPV. The flow cytometry gives interesting results on the cell cycle and the ploidy level; it appears as a good tool for insect cell lines characterization.

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