doi: 10.1007/BF00145029.
A simple freezing medium for serum-free cultured cells
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- PMID: 22359122
- DOI: 10.1007/BF00145029
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A simple freezing medium for serum-free cultured cells
Cytotechnology.
1988 Jul.
Abstract
Methylcellulose was found to protect serum-free cultured cells from the deleterious effects of freezing and thawing. We have formulated a simple medium suitable for freezing serum-free cultured cells that consists of 0.1% methylcellulose, 10% dimethylsulfoxide, and MEM or any other serum-free culture medium.
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