doi: 10.1073/pnas.87.21.8560.
Initiation of rolling-circle replication in pT181 plasmid: initiator protein enhances cruciform extrusion at the origin
Affiliations
- PMID: 2236066
- PMCID: PMC54996
- DOI: 10.1073/pnas.87.21.8560
Item in Clipboard
Initiation of rolling-circle replication in pT181 plasmid: initiator protein enhances cruciform extrusion at the origin
Proc Natl Acad Sci U S A.
1990 Nov.
Abstract
Plasmid pT181 DNA secondary structures have been analyzed in vitro by nuclease S1 digestion and in vivo by bromoacetaldehyde treatment. A cruciform structure occurring at the pT181 replication origin in vitro is greatly enhanced by the binding of the plasmid-encoded initiator protein RepC. In vivo a DNA secondary structure also existed in the replication origin. Its frequency of formation was correlated with efficiency of RepC utilization. These data suggest that cruciform extrusion at the origin is involved in initiation of pT181 rolling-circle replication. A neighboring DNA structure influences the conformation of the origin in vivo.
Similar articles
-
Specificity of RepC protein in plasmid pT181 DNA replication.J Biol Chem. 1990 Feb 25;265(6):3484-8. J Biol Chem. 1990. PMID: 2303456
-
An 18-base-pair sequence is sufficient for termination of rolling-circle replication of plasmid pT181.J Bacteriol. 1996 Sep;178(17):5222-8. doi: 10.1128/jb.178.17.5222-5228.1996. J Bacteriol. 1996. PMID: 8752341 Free PMC article.
-
The replication initiator protein of plasmid pT181 has sequence-specific endonuclease and topoisomerase-like activities.Proc Natl Acad Sci U S A. 1985 Oct;82(20):6845-9. doi: 10.1073/pnas.82.20.6845. Proc Natl Acad Sci U S A. 1985. PMID: 2995991 Free PMC article.
-
DNA-protein interactions during the initiation and termination of plasmid pT181 rolling-circle replication.Prog Nucleic Acid Res Mol Biol. 2003;75:113-37. doi: 10.1016/s0079-6603(03)75004-1. Prog Nucleic Acid Res Mol Biol. 2003. PMID: 14604011 Review.
-
Plasmid rolling-circle replication: highlights of two decades of research.Plasmid. 2005 Mar;53(2):126-36. doi: 10.1016/j.plasmid.2004.12.008. Epub 2005 Jan 22. Plasmid. 2005. PMID: 15737400 Review.
Cited by
-
Palindrome regeneration by template strand-switching mechanism at the origin of DNA replication of porcine circovirus via the rolling-circle melting-pot replication model.J Virol. 2004 Sep;78(17):9016-29. doi: 10.1128/JVI.78.17.9016-9029.2004. J Virol. 2004. PMID: 15308698 Free PMC article.
-
Rep-mediated nicking of the adeno-associated virus origin requires two biochemical activities, DNA helicase activity and transesterification.J Virol. 1999 Nov;73(11):9325-36. doi: 10.1128/JVI.73.11.9325-9336.1999. J Virol. 1999. PMID: 10516041 Free PMC article.
-
In vitro cleavage and joining at the viral origin of replication by the replication initiator protein of tomato yellow leaf curl virus.Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3879-83. doi: 10.1073/pnas.92.9.3879. Proc Natl Acad Sci U S A. 1995. PMID: 7732000 Free PMC article.
-
Identification of Multiple Replication Stages and Origins in the Nucleopolyhedrovirus of Anticarsia gemmatalis.Viruses. 2019 Jul 15;11(7):648. doi: 10.3390/v11070648. Viruses. 2019. PMID: 31311127 Free PMC article.
-
Origin recognition specificity in pT181 plasmids is determined by a functionally asymmetric palindromic DNA element.EMBO J. 1993 Jan;12(1):45-52. doi: 10.1002/j.1460-2075.1993.tb05630.x. EMBO J. 1993. PMID: 8428593 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
