Urinary tract infections caused by staphylococcus aureus DNA in comparison to the candida albicans DNA

Abstract

Background: Bacterial DNA released upon bacterial autolysis or killed by antibiotics, hence, many inflammatogenic reactions will be established leading to serious tissue damage.

Aim: the present work aimed to elucidate the histopathological changes caused by prokaryotic (bacterial) DNA and eukaryotic (candidal) DNA.

Materials and methods: twenty one Staphylococcus aureus and 36 Candida albicans isolates were isolated from UTI patients. Viable cells and DNA of the highest antibiotic sensitive isolates were injected, intraurethraly, in mice. Results were evaluated via histopathological examination.

Results: Mildest reactions were obtained from mice challenged with viable C. albicans compared with those challenged with viable S. aureus. Dose-dependent histological changes were observed for both eukaryotic and prokaryotic DNA. However, the eukaryotic C. albicans DNA developed less intense histological changes than S. aureus DNA.

Conclusion: microbial DNA has the ability to cause damage in murine renal system. Nevertheless, bacterial DNA caused more intense damage than candidal DNA.

Keywords: DNA; Staphylococcus aureus; UTI; candida albicans.

Fig. 1

a: histological section of control mouse kidney 3 days after challenged with 0.1ml of saline shows a normal structure appearance of glomeruli (G) and renal tubules (T). b: The histological section of control mouse urinary bladder 3 days after challenged with 0.1ml of saline shows a normal structure appearance of urinary epithelial lining mucosa (E) and subepithelial lining layer (SE). H & E (X200).

Fig. 2

a: Histological section of mouse kidney 3 days after challenged with 0.1 ml of 1.5 × 10⁸ cfu/ml of S. aureus S11 shows congestion (CO) ), inflammatory cells infiltration (IC) and degenerative changes of renal tubules (D). b: The histological section of mouse urinary bladder 3 days after challenged with 0.1ml of 1.5 ×10⁸ cfu/ml of S. aureus S11 shows edema (O) with moderate inflammatory cells infiltration (IC) and focal mucosal surface epithelial ulceration (FU). c: The histological section of mouse kidney 3 days after challenged with 0.1ml of 1 × 10⁸ cfu/ml of C. albicans C3 shows congestion (CO) and no obvious histological changes. d: The histological section of mouse urinary bladder 3 days after intraurethrally challenged with 0.1ml of 1×10⁸ cfu/ml of C. albicans C3 shows mild infiltration of inflammatory cells (IC) with edema (O) in subepithelial lining layer. H & E (X200).

Fig. 3

a: The histological section of mouse kidney 3 days after challenged with 0.1ml of 50 μg/100 μl of S. aureus S11 DNA showing congestion (CO), degenerative and necrosis (DN) of renal tubule with severe infiltration of inflammatory cells (IC). b: The histological section of mouse urinary bladder 3 days after challenged with 0.1ml of 50 μg/100 μl of S. aureus S11 DNA showing damage of mucosal wall (D) with severe inflammatory cells infiltration (IC). c: The histological section of mouse kidney 3 days after challenged with 0.1ml of 40 μg/100 μl of S. aureus S11 DNA showing congestion (CO) of blood vessel with degenerative changes (D) of renal tubules. d: The histological section of mouse urinary bladder 3 days after challenged with 0.1ml of 40 μg/100 μl of S. aureus S11 DNA showing mild edema (O) and infiltration of inflammatory cells (IC) with sloughing (S) of mucosal epithelial surface. e: The histological section of mouse kidney 3 days after challenged with 0.1ml of 30 μg/100 μl of S. aureus S11 DNA showing congestion (CO) and mild degenerative changes (D) of renal tubules with mild inflammatory cells infiltration (IC). f: The histological section of mouse urinary bladder 3 days after challenged with 0.1ml of 30 μg/100 μl of S. aureus S11 DNA showing edema (O) with infiltration of inflammatory cells (IC). g: The histological section of mouse kidney 3 days after challenged with 0.1ml of 50 μg/100 μl of C. albicans C3 DNA showing congestion (CO) with moderate inflammatory cells infiltration (IC). h: The histological section of mouse urinary bladder 3 days after challenged with 0.1ml of 50 μg/100 μl of C. albicans C3 DNA showing edema (O) with mild inflammatory cells infiltration (IC). i: The histological section of mouse kidney 3 days after challenged with 0.1ml of 40 μg/100 μl of C. albicans C3 DNA showing congestion (CO) of blood vessels. j: The histological section of mouse urinary bladder 3 days after challenged with 0.1ml of 40 μg/100 μl of C. albicans C3 DNA showing mild inflammatory cells (IC) infiltration and edema (O). H & E (X200).

Fig. 4

Effect of DNA concentrations on histological changes severity