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Comparative Study
. 2012 Apr;89(1):38-40.
doi: 10.1016/j.mimet.2012.02.006. Epub 2012 Feb 16.

A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay

K Elwin  1 , G Robinson, S J Hadfield, H V Fairclough, M Iturriza-Gómara, R M Chalmers
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Comparative Study

A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay

K Elwin et al. J Microbiol Methods. 2012 Apr.

Abstract

Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P=0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. However, direct extraction is cheaper, and amenable to automation.

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