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. 2012 Jan;15(1):32-5.
doi: 10.4103/0972-0707.92603.

Comparative evaluation of apical extrusion of bacteria using hand and rotary systems : An in vitro study

Sheetal B Ghivari  1 , Girish C Kubasad, Preethi Deshpande
Affiliations

Comparative evaluation of apical extrusion of bacteria using hand and rotary systems : An in vitro study

Sheetal B Ghivari et al. J Conserv Dent. 2012 Jan.

Abstract

Aim: To evaluate the bacteria extruded apically during root canal preparation using two hand and rotary instrumentation techniques.

Materials and methods: Eighty freshly extracted mandibular premolars were mounted in bacteria collection apparatus. Root canals were contaminated with the pure culture of Enterococcus fecalis (ATCC 29212) and dried at 37°C for 24 h. Bacteria extruded were collected, incubated in brain heart infusion agar for 24 h at 36°C and the colony forming units (CFU) were counted.

Statistical analysis: The mean number of colony forming units were calculated by One-way ANOVA and comparison between the groups made by multiple comparison (Dunnet D) test.

Results: The step-back technique extruded highest number of bacteria in comparison to other hand and rotary Ni-Ti systems.

Conclusion: Under the limitation of this study all hand and rotary instrumentation techniques extruded bacteria. Among all the instrumentation techniques step-back technique extruded more number of bacteria and K-3 system the least. Further in vivo research in this direction could provide more insight into the biologic factors associated and focus on bacterial species that essentially play a major role in post instrumentation flare-ups.

Keywords: Apical extrusion; bacteria; hand and rotary systems.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Bacteria collection apparatus
See this image and copyright information in PMC

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