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. 2011 Nov 22;5 Suppl 8(Suppl 8):P53.
doi: 10.1186/1753-6561-5-S8-P53. eCollection 2011.

Towards human central nervous system in vitro models for preclinical research: strategies for 3D neural cell culture

Daniel Simão  1 , Inês Costa  1 , Margarida Serra  1 , Johannes Schwarz  2 , Catarina Brito  1 , Paula M Alves  1
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Towards human central nervous system in vitro models for preclinical research: strategies for 3D neural cell culture

Daniel Simão et al. BMC Proc. .
No abstract available

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Figures

Figure 1
Figure 1
Effect of inoculum concentration on aggregate concentration (A), aggregate size (B) and viability (C), evaluated using a double stain viability test: fluorescein diacetate (live, green); propidium iodide (dead, red). Cells were cultured in shake flasks with inoculum concentrations of 2 and 4x105 cell/mL. Error bars denote standard deviation of average of 3 independent experiments. *** indicates significant difference (P<0.001) in aggregate diameter by one-way ANOVA analysis with a Tukey’s post-hoc multiple comparison test.
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