doi: 10.1002/anie.201108122.
Epub 2012 Feb 28.
The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the α-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK
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- PMID: 22374809
- DOI: 10.1002/anie.201108122
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The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the α-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK
Angew Chem Int Ed Engl.
.
Abstract
Mystery solved: using heterologous expression, the activities of two enzymes exclusively belonging to the kanamycin biosynthetic pathway have been identified in vitro. A distinctive reaction mechanism to produce kanamycin is proposed and the previously unknown catalytic deamination activity of KanJ dioxygenase is uncovered.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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