[The cloning of "residual" DNA and the determination of the primary structure for the fragments of the barley-specific family of tandem repetitive sequences]
[Article in
Russian]
- PMID: 2238099
Item in Clipboard
[The cloning of "residual" DNA and the determination of the primary structure for the fragments of the barley-specific family of tandem repetitive sequences]
[Article in
Russian]
Tsitol Genet.
1990 May-Jun.
Abstract
High molecular weight "relic" DNA fraction can be separated from the bulk of barley DNA digested with different restriction enzymes by agarose gel electrophoresis. The majority of AluI-relic DNA clones contained barley simple sequence satellite DNA and other families of repetitive DNA. The clones representing HvRT family were sequenced. This family was found to be barley-specific. It is composed of tandemly arranged 118-bp monomers and presents in 7 x 10(5) copies in barley genome.