Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Dec;38(4):186-92.
doi: 10.5653/cerm.2011.38.4.186. Epub 2011 Dec 31.

Application of two different synthetic sequential media for the human IVF-ET program: a prospective, randomized, and comparative study

Jeong Yoon  1 , Hye-Jin Yoon, Kyoung-Mi Juhn, Jin-Kyung Ko, San-Hyun Yoon, Yong Ko, Jin-Ho Lim
Affiliations

Application of two different synthetic sequential media for the human IVF-ET program: a prospective, randomized, and comparative study

Jeong Yoon et al. Clin Exp Reprod Med. 2011 Dec.

Abstract

Objective: Since IVF program was first established, various types of media and culture systems have been developed either in-house or commercially. The aim of this study was to compare the efficacy of in-house Maria Research Center (MRC) media to that of commercially available Sydney IVF media in human day 3 embryo transfer cycles.

Methods: Three hundred sixty nine couples were included in this prospective, randomized, and comparative study. All couples undergoing IVF treatment at the Maria Fertility Hospital were randomly assigned to either Sydney IVF (n=178) or MRC (n=191) media.

Results: No difference was observed between the MRC media and Sydney IVF media groups with respect to fertilization rate (74.4% vs. 75.5%). The clinical pregnancy and implantation rates of MRC media (47.1% and 20.0%, respectively) were also similar to those of Sydney IVF media (44.4% and 19.4%, respectively). However, the proportion of embryos with good quality on day 3 was significantly higher in the MRC media group than the Sydney IVF media group (50.2% vs. 43.2%) (p<0.05).

Conclusion: MRC media were as effective as Sydney IVF media for sustaining embryo development and pregnancy rates. The present study implies that MRC media can be a suitable alternative to commercially available media for human IVF-ET program.

Keywords: Day 3 embryo transfer; Human; In vitro fertilization-embryo transfer; MRC media; Sydney IVF media.

PubMed Disclaimer

Conflict of interest statement

No potential conflict of interest relevant to this article was reported.

Figures

Figure 1
Figure 1
Day 3 good quality embryo rate among batches of sequential MRC media compared to Sydney IVF media.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Yoon HG, Yoon SH, Son WY, Kim JG, Im KS, Lim JH. Alternative embryo transfer on day 3 or day 5 for reducing the risk of multiple gestations. J Assist Reprod Genet. 2001;18:262–267. - PMC - PubMed
    1. Yoon HG, Yoon SH, Son WY, Lee SW, Park SP, Im KS, et al. Pregnancies resulting from in vitro matured oocytes collected from women with regular menstrual cycle. J Assist Reprod Genet. 2001;18:325–329. - PMC - PubMed
    1. Son WY, Yoon SH, Lee SW, Ko Y, Yoon HG, Lim JH. Blastocyst development and pregnancies after IVF of mature oocytes retrieved from unstimulated patients with PCOS after in-vivo HCG priming. Hum Reprod. 2002;17:134–136. - PubMed
    1. Son WY, Yoon SH, Lim JH. Effect of gonadotrophin priming on in-vitro maturation of oocytes collected from women at risk of OHSS. Reprod Biomed Online. 2006;13:340–348. - PubMed
    1. Staessen C, Janssenswillen C, De Clerck E, Van Steirteghem A. Controlled comparison of commercial media for human in-vitro fertilization: Ménézo B2 medium versus Medi-Cult universal and BM1 medium. Hum Reprod. 1998;13:2548–2554. - PubMed