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. 2012 Feb;78(1):51-6.
doi: 10.1590/s1808-86942012000100008.

Influence of sealant fibrin on the wound healing of the pigs vocal folds

[Article in English, Portuguese]
Karla Palma Portes  1 , André de Campos Duprat, Carmen Lucia Penteado Lancellotti, Leonardo Silva, Flávia Coelho de Souza
Affiliations

Influence of sealant fibrin on the wound healing of the pigs vocal folds

[Article in English, Portuguese]
Karla Palma Portes et al. Braz J Otorhinolaryngol. 2012 Feb.

Abstract

Fibrin sealants or fibrin glue are products made from human plasma proteins, which mimic the final pathway of the coagulation cascade. Its application to stimulate the healing process has been a topic of debate in the literature. The use of fibrin sealants in phonosurgery has been empirical; there have been no studies that investigate the action of fibrin sealant in Reinke's space.

Aim: To evaluate the effect of fibrin glue in healing of the vocal folds of pigs after surgical manipulation.

Materials and methods: This was a prospective and experimental study. Six animals had both vocal folds incised. Sealant was applied in one of them; the other served as a control. After three months, the animals were sacrificed and a collagen count was carried out.

Results: The side on which glue was applied had an average of 27.8% against 20.4% of the side without glue.

Conclusion: The collagen concentration in the samples where the fibrin sealant was applied was significantly higher compared to samples without glue. Thus, the presence of a fibrin sealant stimulates fibrogenesis in this tissue.

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Figures

Figure 1
Figure 1
Mouth opener specifically developed for laryngoscopy in pigs (Pontes et al, 2007).
Figure 2
Figure 2
View by using the mouth opener for carrying out the procedure.
Figure 3
Figure 3
Incision on the vocal fold.
Figure 4
Figure 4
PVD pig 76 – Fibrin sealant was applied to this vocal fold; note intense fibroplasia (stained red)
Figure 5
Figure 5
PVD Pig 79 – Note the intense red; collagen fibers are stained by picrosirius red, and contrast with the epithelial and gland tissues (blue).
Chart 1
Chart 1
Comparison of collagen concentrations among vocal folds with and without glue; analysis of the Complete and Simulation groups.
See this image and copyright information in PMC

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