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Comparative Study
. 2012 May;19(5):817-9.
doi: 10.1128/CVI.05687-11. Epub 2012 Mar 7.

New enzyme-linked immunosorbent assay for detection of antibodies against hepatitis delta virus using a hepatitis delta antigen derived from a Taiwanese clone and comparison to the Abbott radioimmunoassay

Yung-Bin Kuo  1 , Mei Chao, Yi-Hsuan Lee, Chau-Ting Yeh, Err-Cheng Chan
Affiliations
Comparative Study

New enzyme-linked immunosorbent assay for detection of antibodies against hepatitis delta virus using a hepatitis delta antigen derived from a Taiwanese clone and comparison to the Abbott radioimmunoassay

Yung-Bin Kuo et al. Clin Vaccine Immunol. 2012 May.

Abstract

An anti-hepatitis delta (HD) enzyme-linked immunosorbent assay (ELISA) using a specific recombinant hepatitis delta antigen derived from a local dominant hepatitis delta virus (hepatitis D virus; HDV) strain in Taiwan has been established. The detection efficiency of this assay was comparable to that of the commercially available Abbott anti-HD radioimmunoassay (RIA) and could be useful in routine laboratory diagnoses of HDV infection.

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Figures

Fig 1
Fig 1
Expression and purification of recombinant HDAg. Lane 1, crude extract from E. coli [BL21(DE3)pLysS] transfected with the expression plasmid (pET15b-SMII); lane 2, purified rHDAg protein. The arrow marks the position of the rHDAg protein fragment (23 kDa). M, molecular size ladder.
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