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. 2012 Mar;86(3):441-5.
doi: 10.4269/ajtmh.2012.11-0548.

Molecular epidemiologic characterization of Enterocytozoon bieneusi in HIV-infected persons in Benin City, Nigeria

Frederick O Akinbo  1 , Christopher E Okaka, Richard Omoregie, Theressa Dearen, Eucaris Torres Leon, Lihua Xiao
Affiliations

Molecular epidemiologic characterization of Enterocytozoon bieneusi in HIV-infected persons in Benin City, Nigeria

Frederick O Akinbo et al. Am J Trop Med Hyg. 2012 Mar.

Abstract

Molecular characterization of Enterocytozoon bieneusi has led to better understanding of microsporidiosis transmission in humans. This study aimed to detect and genotype E. bieneusi in human immunodeficiency virus (HIV)-infected persons. Stool specimens were collected from 463 HIV-infected patients and analyzed for E. bieneusi by polymerase chain reaction (PCR) and DNA sequence analysis of the internal transcribed spacer. E. bieneusi was detected in 77 HIV patients. CD4 cell counts < 200 cells/μL was associated with E. bieneusi infection (P = 0.09). E. bieneusi was significantly associated with weight loss (P < 0.0001), diarrhea (P = 0.006), fever (P < 0.0001), not being married (P < 0.0001), and flush type of toilet (P = 0.0007). Six known genotypes of D, A, IV, CAF2, EbpA, and Peru 8 in 31, 22, 14, 2, 1, and 1 patients, respectively, five novel genotypes of E. bieneusi, and one infection with mixed genotypes were observed in this study. Three of the novel genotypes were genetically distant to the genotypes commonly found in humans.

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Figures

Figure 1.
Figure 1.
Phylogenetic relationship among known E. bieneusi genotypes and new genotypes identified in AIDS patients in Nigeria as indicated by a neighbor-joining analysis of the ITS sequence, based on genetic distances calculated by the Kimura 2-parameter model. Bootstrap values greater than 50% from 1,000 replicates are shown on nodes. The group terminology for the clusters are based on Sulaiman et al., 2003 and Thellier and Breton, 2008. Each sequence from GenBank is identified by the accession number, host origin, and the original genotype designation. Genotypes in these studies are designated as Nig1- to Nig5.
See this image and copyright information in PMC

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