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. 2012 May;50(5):1758-61.
doi: 10.1128/JCM.00155-12. Epub 2012 Mar 7.

Molecular fingerprinting of Mycobacterium abscessus strains in a cohort of pediatric cystic fibrosis patients

Kathryn A Harris  1 , Dervla T D Kenna, Cornelis Blauwendraat, John C Hartley, Jane F Turton, Paul Aurora, Garth L J Dixon
Affiliations

Molecular fingerprinting of Mycobacterium abscessus strains in a cohort of pediatric cystic fibrosis patients

Kathryn A Harris et al. J Clin Microbiol. 2012 May.

Abstract

Forty-one Mycobacterium abscessus complex isolates from 17 pediatric cystic fibrosis (CF) patients were typed using a novel variable-number tandem repeat (VNTR) scheme and an automated repetitive-PCR (rep-PCR) system. Both VNTR and rep-PCR typing methods differentiate between members of the M. abscessus complex. The isolates from individual patients are indistinguishable, and the data strongly suggest that individual CF patients are persistently infected with one strain and also suggests that different CF patients can harbor the same strain.

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Figures

Fig 1
Fig 1
Diversilab rep-PCR dendrogram constructed using the Kullback-Leibler method. The VNTR profile is also displayed, and identical profiles are indicated by the color coding. The first part of the sample ID is the patient ID (e.g., gos-1). Clusters of isolates from different patients that are indistinguishable by both typing methods using a 90% similarity coefficient are indicated.
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