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. 2012 May;50(5):1721-4.
doi: 10.1128/JCM.00531-12. Epub 2012 Mar 7.

Characterization of microbiota of root canal-treated teeth with posttreatment disease

Isabela N Rôças  1 , José F Siqueira Jr
Affiliations

Characterization of microbiota of root canal-treated teeth with posttreatment disease

Isabela N Rôças et al. J Clin Microbiol. 2012 May.

Abstract

This study evaluated the microbiota of root canals undergoing retreatment. The most prevalent taxa detected by checkerboard included Propionibacterium species, Fusobacterium nucleatum, streptococci, and Pseudoramibacter alactolyticus. Quantitative real-time PCR detected Enterococcus faecalis and streptococci in 38% and 41% of the cases, comprising 9.76% and 65.78% of the total bacterial counts, respectively. The findings call into question the status of E. faecalis as the main pathogen and suggest that other species can be candidate pathogens associated with persistent/secondary endodontic infections.

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Figures

Fig 1
Fig 1
Stacked bar chart of frequencies of detection and levels of bacterial species/phylotypes in root canal samples of treated teeth with posttreatment apical periodontitis from 42 individuals. Total length of each bar stack indicates percentage of positive samples; i.e., prevalence of bacterial species/phylotypes. Different shades within each bar indicate the percentage of samples containing different levels of the species. ss, subspecies.
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