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. 2012 Mar 13;7(1):6.
doi: 10.1186/1749-8546-7-6.

Metabolomic profiles of myocardial ischemia under treatment with salvianolic acid B

Yonghai Lu  1 , Yue Zheng, Xinru Liu, Xu Liang, Saiming Ngai, Tiejun Li, Weidong Zhang
Affiliations

Metabolomic profiles of myocardial ischemia under treatment with salvianolic acid B

Yonghai Lu et al. Chin Med. .

Abstract

Background: Radix Salvia miltiorrhiza (Danshen) has been used as a principal herb in treating cardiovascular diseases in Chinese medicine. Salvianolic acid B (SA-B), a water-soluble active component of Danshen, was found to have anti-myocardial ischemia (anti-MI) effect. This study aims to investigate mechanisms of SA-B on MI.

Methods: Five conventional Western medicines (isosorbide dinitrate, verapamil, propranolol, captopril and trimethazine) with different mechanisms for treating cardiovascular diseases were selected as positive references to compare with SA-B in changing of the metabolomic profiles in MI rats under treatment. Potential mechanisms of SA-B were further investigated in H9C2 cell line.

Results: The metabolomic profiles between SA-B- and propranolol-treated MI rats were similar, since there was a big overlap between the two groups in the PLS-DA score plot. Finally, it was demonstrated that SA-B exhibited a protective effect on MI mainly by decreasing the concentration of cyclic adenosine monophosphate (cAMP) and Ca2+ and inhibiting protein kinase A (PKA).

Conclusion: SA-B and propanolol exhibited similar metabolomic profiles, indicating that the two drugs might have a similar mechanism.

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Figures

Figure 1
Figure 1
(a) PLS-DA score plot of the metabolic profiles obtained from 6 sham rats (■), 6 MI rats with no treatment (red diamond symbol), and 6 MI rats with SA-B treatment (blue triangle symbol). (R2X = 0.798, R2Y = 0.975, Q2 = 0.858, A = 4, N = 18, K = 160); (b) Validation plot obtained from permutation test (n = 100). R2 is the explained variance, and Q2 is the predictive ability of the model.
Figure 2
Figure 2
PLS-DA score plot of the metabolic profiles obtained from all drug-treated rats: (+) SA-B, (■) propranolol (Δ) isosorbide dinitrate, (○) trimethazine, (*) verapamil, and (▲) captopril. (R2Y = 0.61, R2X = 0.833, Q2 = 0.512, A = 5, N = 36, K = 2700).
Figure 3
Figure 3
Effects of SA-B on H9C2 cell viability.
Figure 4
Figure 4
Effects of SA-B on the cellular calcium concentration in H9C2 cells. ** P < 0.01 compared with anoxia.
Figure 5
Figure 5
Effects of SA-B on the levels of cAMP in H9C2 cells. ** P < 0.01 compared with anoxia.
Figure 6
Figure 6
Effects of SA-B on the PKA activity in H9C2 cell. ** P < 0.01 compared with anoxia.
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