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. 1990 Oct 30;172(2):811-6.
doi: 10.1016/0006-291x(90)90747-b.

Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain

A Verma  1 , D J Hirsch, M R Hanley, O Thastrup, S B Christensen, S H Snyder
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Free article

Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain

A Verma et al. Biochem Biophys Res Commun. .
Free article

Abstract

ATP dependent Ca2+ accumulation into oxalate-loaded rat brain microsomes is potently inhibited by thapsigargin with an IC50 of 2 nM and maximal inhibition at 10 nM. Approximately 15% of the total A23187-releasable microsomal calcium store is insensitive to thapsigargin concentrations up to 100 microM. Inositol-1,4,5-trisphosphate (IP3) maximally inhibits 40% of the net Ca2+ accumulation by whole brain microsomes. Its effects are non-additive with thapsigargin suggesting that the IP3-sensitive Ca2+ pool is a subset of the thapsigargin sensitive Ca2+ pool. Marked regional differences occur in Ca2+ transport rates and sensitivity to both thapsigargin and IP3.

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