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. 2012 Jun;50(6):2089-91.
doi: 10.1128/JCM.00567-12. Epub 2012 Mar 14.

Direct detection of Mycobacterium tuberculosis complex in clinical samples by a molecular method based on GenoQuick technology

Raquel Moure  1 , Miriam Torres, Rogelio Martín, Fernando Alcaide
Affiliations

Direct detection of Mycobacterium tuberculosis complex in clinical samples by a molecular method based on GenoQuick technology

Raquel Moure et al. J Clin Microbiol. 2012 Jun.

Abstract

Several molecular systems for direct detection of Mycobacterium tuberculosis complex (MTBC) have recently been developed. The GenoQuick MTB assay (GQ-MTB) used in this study detected 82 of the 96 (85.4%) samples with MTBC, including 50 of 64 (78.1%) samples with negative acid-fast bacillus smears. Fifteen samples containing nontuberculous mycobacteria were also studied: 13 were GQ-MTB negative, one was positive, and one was indeterminate. GQ-MTB showed good effectiveness for the direct detection of MTBC from clinical samples.

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Figures

Fig 1
Fig 1
Images of the results obtained with the GQ-MTB technique. MTBC, Mycobacterium tuberculosis complex; AC, amplification control; CC, conjugate control. (A) Positive result. Three different possibilities were obtained: all bands were well marked (A.1), the MTBC band was present though faded (A.2), and the amplification control band was absent but the MTBC band was present (A.3). (B) Negative result (MTBC band completely missing). (C) Invalid result (both amplification control and MTBC bands were missing).
See this image and copyright information in PMC

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