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. 2011 Jul;15(3):262-4.

Scintigraphic evaluation of small intestinal transit in the streptozotocin induced diabetic rats

G Durmus-Altun, U Vatansever, S Arzu Vardar, S Altaner, B Dirlik

Scintigraphic evaluation of small intestinal transit in the streptozotocin induced diabetic rats

G Durmus-Altun et al. Hippokratia. 2011 Jul.

Abstract

Aim: Small intestine (SI) transit in the streptozotocin (STZ) induced diabetic rats were examined by using 99mTc-mebrofenin scintigraphy.

Materials and methods: Wistar albino rats (mean body weight: 220±12 g) were studied for both control (n=10) and diabetes mellitus (DM) (n=10) groups. Diabetes was induced by a single intraperitoneal injection of streptozotocin (50 mg kg(-1) body weight. SI transit time was assessed by measuring arrival times of 99mTc-mebrofenin from duodenum to caecum.

Results: The mean transit time of 99mTc- mebrofenin was 67.8±11 min in control group. The mean transit time of SI was prolonged in STZ induced diabetic animals with (111.9±12.5, p=0.01). There was significant correlation between small intestinal transit time and blood glucose level (r: 0.73, p=0.01).

Conclusion: We observed that SI transit was prolonged in diabetic animals using 99mTc- mebrofenin, and additionally this technique is a readily available method for the detection of transit abnormalities in animal experiment.

Keywords: 99mTc- mebrofenin; Small intestine; diabetes mellitus; scintigraphy; streptozotocin.

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Figures

Figure 1
Figure 1. a) 99mTc-mebrofenin scintigraphy images of control animal, including hepatic and intestinal migration phases (5, 20, 40 and 60 min spot images). b) Time-activity curves ROIs over the duodenum (D) and the caecum (C). The respective time points for the appearance of the radioactivity in the separate ROIs were taken as the start and end-point of transit.
Figure 2
Figure 2. The mean transit time of SI (SITT) was prolonged in STZ induced diabetic animals.
Figure 3
Figure 3. There was good correlation between small intestinal transit time (SITT) and blood glucose level (BGL) (r: 0.65, p=0.01).
See this image and copyright information in PMC

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