Estrogen, a female hormone involved in spermatogenesis

Abstract

In mammalian testes, aromatase irreversibly converts androgens (C19 steroid) into estrogens (C18) and is present in the endoplasmic reticulum of numerous tissues. In purified adult rat germ cells (pachytene spermatocytes and round spermatids) we have shown the presence of a functional aromatase (transcript, protein and biological activity) and the estrogen production is roughly identical to that of Leydig cells. In addition, transcripts of aromatase varied according to the germ cell type and the stages of seminiferous epithelium in an adult rat. In contrast with the androgen receptors mainly localized in somatic cells, estrogen receptors (ERs) are described in all testicular cells. Moreover, besides the presence of high affinity ERα and ERβ a rapid membrane effects have been recently reported and we demonstrated that GPR30 (a transmembrane intracellular estrogen receptor) was expressed in adult rat pachytene spermatocytes and in round spermatids. Thus estrogens through both GPR30 and genomic effects are able to activate the rapid signaling cascade, which in turn triggers an apoptotic mitochondrial pathway (via an increase in Bax expression) and a concomitant decrease of cyclin A1 and B1 gene levels as well as in controlling apoptosis and maturation/differentiation of round spermatids. Hence, the role of estrogen (either intracrine, paracrine or autocrine) in spermatogenesis (proliferation, apoptosis, survival and maturation) is now obvious taking into account the simultaneous presence of a biologically active aromatase and the widespread distribution of estrogen receptors especially during the spermiogenesis steps.