Developmental progression of Tetrahymena through the cell cycle and conjugation

Abstract

The ciliate Tetrahymena thermophila can be said to undergo a variety of developmental programs. During vegetative growth, cells coordinate a variety of cell-cycle operations including macronuclear DNA synthesis and a-mitotic fission, micronuclear DNA synthesis and mitosis, cytokinesis and an elaborate program of cortical morphogenesis that replicates the cortical organelles. When starved, cells undergo oral replacement, transformation into fast-swimming dispersal forms or, when encountering cells of a complementary mating type, conjugation. Conjugation involves a 12 hour program of meiosis, mitosis, nuclear exchange and karyogamy, and two postzygotic divisions of the fertilization nucleus. This chapter reviews experimental data exploring the developmental dependencies associated with both vegetative and conjugal development.