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Comment
. 2012 Apr 24;109(17):E989; author reply E990.
doi: 10.1073/pnas.1202000109. Epub 2012 Mar 23.

Imaging of protein synthesis with puromycin

Craig A Goodman, Philippe Pierre, Troy A Hornberger
Comment

Imaging of protein synthesis with puromycin

Craig A Goodman et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
(A) Confocal immunofluorescence imaging of HeLa cells labeled with puromycin for 10 min. Puromycin-labeled proteins detected with antipuromycin 12D10 antibody (magenta) are mostly localized on the endoplasmic reticulum (ER) and in the cytosol as previously described (2, 3). Late endosomes and lysosomes [lysosomal-associated membrane protein 1 (LAMP1); green] are shown to delimit the cells cytoplasm. (B–D) A cross-section of skeletal muscle (tibialis anterior) from a puromycin-treated mouse was subjected to immunohistochemistry for puromycin-labeled proteins (B) and the ribosomal S6 protein (C) as previously described (4). (D) A merge of the images from B (red) and C (green) reveals extensive colocalization between the signals for puromycin and the ribosomal S6 protein. Furthermore, the most prominent signal was consistently found near the periphery of the individual fibers, with a more diffuse reticular signal found in the intermyofibrillar regions.
See this image and copyright information in PMC

Comment on

References

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