Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jul;18(1):71-6.
doi: 10.1007/BF00321118.

The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG-negative mutant strain

F Gruber  1 , J Visser, C P Kubicek, L H de Graaff
Affiliations

The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG-negative mutant strain

F Gruber et al. Curr Genet. 1990 Jul.

Abstract

Six uridine auxotroph mutants of Trichoderma reesei QM 9414 were isolated by resistance to 5-fluoroorotic acid and one strain was identified as OMP-decarboxylase negative (pyr-) by a radiometric enzyme assay. Transformation to uridine prototrophy was achieved with the pyr4 gene of Neurospora crassa (up to 1500 transformants/micrograms) and with pyrA of Aspergillus niger (700-800 transformants/micrograms). In many transformants the PYR+ function seems to be present as extrachromosomal DNA. There is evidence for a correlation between the stability of transformants and integration of the vector in the genome whereas unstable transformants are obtained when autonomous replication of the plasmid occurs.

PubMed Disclaimer

References

    1. Mol Cell Biol. 1983 Aug;3(8):1430-9 - PubMed
    1. Mol Gen Genet. 1983;190(3):403-5 - PubMed
    1. Biochem Soc Trans. 1985 Apr;13(2):414-6 - PubMed
    1. J Bacteriol. 1985 Jan;161(1):265-71 - PubMed
    1. Curr Genet. 1988 Apr;13(4):315-21 - PubMed

MeSH terms

LinkOut - more resources