Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Mar 29:8:39.
doi: 10.1186/1746-6148-8-39.

Genetically divergent methicillin-resistant Staphylococcus aureus and sec-dependent mastitis of dairy goats in Taiwan

Chishih Chu  1 , Changyou Yu, Yanhaui Lee, Yaochi Su
Affiliations

Genetically divergent methicillin-resistant Staphylococcus aureus and sec-dependent mastitis of dairy goats in Taiwan

Chishih Chu et al. BMC Vet Res. .

Abstract

Background: Widespread in the environment, Staphylococcus spp. infect animals and humans as normal flora or pathogens. By extending our recent report of multi-drug resistant (MDR) S. aureus in dairy goats, this study investigated the staphylococcal infection and characterized the MDR-S. aureus and methicillin-resistant S. aureus (MRSA) isolates collected from goats in 2008 to elucidate the appearance of MRSA in goats and the mastitis associated staphylococcus enterotoxin (SE) types. A total of 555 samples were collected from six goat parts and three environmental sources among four dairy goat farms in southern Taiwan. Coagulase-positive and negative Staphylococcus spp. (CPS and CNS, respectively) were also identified. Furthermore, predominant SE genes of nine enterotoxin genes sea through sej along with antimicrobial resistance and genetic variations were determined.

Results: In total, 137 staphylococcal strains were identified and found predominantly in milk, and in the vagina, anus, and nasal cavity. The most prevalent species was S. lentus, followed by S. aureus, S. epidermidis, and S. xylosus. Enterotoxin genes were not identified in any CNS isolates, however sec and see were identified only in S. aureus associated with mastitis in goat. In compared to the isolates from 2006 to 2007, 27 S. aureus isolates from 2008 were found to be more resistant to ampicillin, cephalothin, oxacillin, oxytetracycline, penicillin G, and tetracycline. Eleven MRSA isolates were identified and belonged to SCCmec type III (nine isolates) as the major type and SCCmec type II (two isolates). These MRSA isolates revealed pulse-field gel electrophoresis (PFGE) pattern A (five isolates), C (one isolate), and D (one isolate) of human isolates. The other two isolates without pulsotypes belonged to ST59.

Conclusion: The prevalence and infection sites of CNS differed from those of CPS. Genetic analyses indicated that genetic divergence, possible zoonotic transfer of MRSA, and the involvement of sec as important virulence factors for of S. aureus that lead to mastitis in goats.

PubMed Disclaimer

Figures

Figure 1
Figure 1
PFGE patterns of SmaI-digested DNA isolated from clinical S. aureus goat isolates. Lanes 1, 4 and 7: pulsotype I; lane 2: pulsotype II, lane 3: pulsotype III, lane 5: pulsotype IV, lane 6: pulsotype V; lane 8: pulsotype VIA; and lane 9: pulsotype VIB. M: λ DNA marker.
Figure 2
Figure 2
Phylogenetic tree of sec genes from eight Staphylococcus aureus isolates from goats with mastitis. The tree was constructed using the Megalign program of the Lasergene software. Scale bar represents number of nucleotide substitutions per? bases.
See this image and copyright information in PMC

References

    1. Resch M, Nagel V, Hertel C. Antibiotic resistance of coagulase-negative staphylococci associated with food and used in starter cultures. Int J Food Microbiol. 2008;127:99–104. doi: 10.1016/j.ijfoodmicro.2008.06.013. - DOI - PubMed
    1. Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC, Maguire D. In: Veterinary Microbiology and Microbial Disease. 1. Quinn PJ, editor. USA: Blackwell Science Ltd; 2002. Staphylococcus species; pp. 43–48.
    1. Abigail AS, Whitt DD. Bacterial Pathogenesis: A Molecular Approach. Washington: ASM Press; 2001.
    1. Taponen S, Pyorala S. Coagulase-negative staphylococci as cause of bovine mastitis-not so different from Staphylococcus aureus? Vet Microbiol. 2009;134:29–36. doi: 10.1016/j.vetmic.2008.09.011. - DOI - PubMed
    1. Krimmer V, Merkert H, von Eiff C, Frosch M, Eulert J, Löhr JF, Hacker J, Ziebuhr W. Detection of Staphylococcus aureus and Staphylococcus epidermidi in clinical samples by 16S rRNA-directed in situ hybridization. J Clin Microbiol. 1999;37:2667–2673. - PMC - PubMed

Publication types

LinkOut - more resources