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. 2012 Jul;61(Pt 7):990-997.
doi: 10.1099/jmm.0.042499-0. Epub 2012 Mar 30.

Identification of porcine rotavirus-like genotype P[6] strains in Taiwanese children

Kao-Pin Hwang  1 , Fang-Tzy Wu  2   3 , Krisztián Bányai  4 , Ho-Sheng Wu  5   3 , Dustin Chen-Fu Yang  3 , Yhu-Chering Huang  6 , Jen-Shiou Lin  7 , Chao Agnes Hsiung  8 , Jason C Huang  2 , Baoming Jiang  9 , Jon R Gentsch  9
Affiliations

Identification of porcine rotavirus-like genotype P[6] strains in Taiwanese children

Kao-Pin Hwang et al. J Med Microbiol. 2012 Jul.

Abstract

The molecular characterization of genotype P[6] rotavirus strains collected from children admitted to hospital with acute dehydrating diarrhoea during a 6-year surveillance period in Taiwan is described in this study. In total, three G4P[6] strains, one G5P[6] and one G12P[6] were characterized by sequencing and phylogenetic analysis of the VP4, VP7, VP6 and NSP4 genes. Whilst all four genes of the single Taiwanese G12P[6] strain clustered with the respective genes of globally common human rotavirus strains, the G4 and G5 strains showed remarkable similarities to porcine rotavirus strains and putative porcine-origin human P[19] strains reported previously from Taiwan. The overall proportion of porcine rotavirus-like strains in Taiwan remains around 1 % among hospitalized children; however, the circulation and sporadic transmission of these heterotypic strains from pigs to humans could pose a public-health concern. Therefore, continuation of strain monitoring is needed in the vaccine era to detect any possible vaccine breakthrough events associated with the introduction of such heterologous rotavirus strains.

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Figures

Fig. 1.
Fig. 1.
Nucleotide sequence-based neighbour-joining phylogenetic tree of the P[6] VP4 gene. Arrows indicate the Taiwanese G4P[6], G5P[6] and G12P[6] strains. Bootstrap values (500 replicates) >60% are indicated. Bar, 0.05 nucleotide substitutions per site.
Fig. 2.
Fig. 2.
Nucleotide sequence-based neighbour-joining phylogenetic trees of the G4 (a), G5 (b) and G12 (c) VP7 genes. Arrows indicate the Taiwanese genotype P[6] strains. Bootstrap values (500 replicates) >60% are indicated. Bars, 0.02 nucleotide substitutions per site.
Fig. 3.
Fig. 3.
Nucleotide sequence-based neighbour-joining phylogenetic trees of the VP6 (a) and NSP4 (b) genes of Taiwanese P[6] strains. Arrows indicate the Taiwanese P[6] strains. Bootstrap values (500 replicates) >60% are indicated. In (a), the genotypes are indicated. Bars, 0.02 nucleotide substitutions per site.
See this image and copyright information in PMC

References

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