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. 2012 Feb 8;56(1):e7.
doi: 10.4081/ejh.2012.e7.

NADPH-d activity in rat thymus after the application of retinoid acid

F Dorko  1 , T Spakovská, K Lovasová, P Patlevič, D Kluchová
Affiliations

NADPH-d activity in rat thymus after the application of retinoid acid

F Dorko et al. Eur J Histochem. .

Abstract

The aim of this work was to determine the localization of nicotinamide-adenine dinucleotide phosphate-diaphorase (NADPH-d) activity as the marker for synthesis of nitric oxide synthase (NOS) in the rat thymus after the application of retinoid acid (RA) on 1st, 7th, 14th and 21st days of gestation. The given results can build the basis for understanding of the role of NOS in rat thymus. NADPH-d positive cells were represented with dark-blue color and were localized on corticomedullar junction of the thymus. These cells were of different intensity of coloring and were shaped in oval, circle or irregular forms. NADPH-d positive nerve fibers were observed in perivascular topography. They were marked more strongly in the case of control group. The result of application of RA to gravid rats was that the birth weights of newborn rats and their thymuses were smaller, but without statistically significance.

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Figures

Figure 1
Figure 1
Experimental group: newborn rats thymus after prenatal application of retinoid acid (1st day after delivery). NADPH-d positive nerve fibers are weakly marked in perivascular topography. No cell bodies were observed.
Figure 2
Figure 2
Control group: newborn rats thymus without prenatal application of retinoid acid (1st day after delivery). Irregularly shaped cells with deeply blue colored protuberances localized in the thymic medulla.
Figure 3
Figure 3
Experimental group: newborn rats thymus after prenatal application of retinoid acid (14th day after delivery). Rich represented NADPH-d positive cells on the corticomedullar junction. The presence of NADPH-d positive neural fibers in perivascular topography was observed.
Figure 4
Figure 4
Control group: newborn rats thymus without prenatal application of retinoid acid (14th day after delivery). There are more NADPH-d positive neural fibers in the case of control group not only in the perivascular topography but also in the thymus parenchyma. The control group had higher number of irregular cells.
Figure 5
Figure 5
Experimental group: newborn rats thymus after prenatal application of retinoid acid (21st day after delivery). Strongly marked intensity of NADPH-d positive cells in thymus parenchyma was observed. Perivascular nerve fibers are weakly marked (experiment group).
Figure 6
Figure 6
Control group: newborn rats thymus without prenatal application of retinoid acid (21st day after delivery). Strongly marked NADPH-d nerve fibers in perivascular topography and in thymic connective tissue septum.
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