Hypoglycemic and Hypolipidemic Effects of Ethanolic Extract of Mirabilis jalapa L. Root on Normal and Diabetic Mice

Abstract

The present study investigated the insulin sensitivity, hypoglycemic, and hypolipidemic activities of ethanolic extract of Mirabilis jalapa L. root (EEM) in normal and diabetic mice. After induction of diabetes with streptozotocin, both normal and diabetic mice were singly or repeatedly for 28 days administrated with EEM at doses of 2, 4, 8 g/kg, respectively. Before induction of diabetes, mice were administrated with EEM at doses of 2, 4, 8 g/kg for 14 days and were injected with streptozotocin and continued on EEM administration for another 28 days. Both after and before induction of diabetes, repeated administration with 4, 8 g/kg EEM continually lowered blood glucose level, decreased serum insulin level and improved insulin sensitivity index, and lowered serum total cholesterol, triglyceride levels and triglyceride content in liver and skeletal muscle, and increased glycogen content in these tissues; but repeated administration had no influence on those indexes of normal mice. Single administration with EEM (4, 8 g/kg) showed hypoglycemic effect in oral glucose tolerance test in normal and diabetic mice. Single administration with EEM had no hypoglycemic and hypolipidemic effects on normal and diabetic mice. These results suggest that EEM possesses both potential insulin sensitivity, hypoglycemic, and hypolipidemic effects on diabetes.

Figure 1

Representative high-performance liquid chromatography chromatograms of EEM and trigonelline (R _t = 19.725).

Figure 2

Effects of single and repeated EEM administration on blood glucose level in normal and diabetic mice. Data are expressed as mean ± SD, n = 10 mice per group. *P < 0.01, compared to normal control. ^# P < 0.01, compared to diabetic control. ^▼ P < 0.01, compared to before administration.

Figure 3

Effects of EEM on oral glucose tolerance test in normal and diabetic mice. Data are expressed as mean ± SD, n = 10 mice per group. *P < 0.01, compared to normal control. ^# P < 0.01, compared to diabetic control.