Songbird chemosignals: volatile compounds in preen gland secretions vary among individuals, sexes, and populations

Abstract

Chemical signaling has been documented in many animals, but its potential importance in avian species, particularly songbirds, has received far less attention. We tested whether volatile compounds in the preen oil of a songbird (Junco hyemalis) contain reliable information about individual identity, sex, or population of origin by repeated sampling from captive male and female juncos originating from 2 recently diverged junco populations in southern California. One of the populations recently colonized an urban environment; the other resides in a species-typical montane environment. The birds were field-caught as juveniles, housed under identical conditions, and fed the same diet for 10 months prior to sampling. We used capillary gas chromatography-mass spectrometry to quantify the relative abundance of 19 volatile compounds previously shown to vary seasonally in this species. We found individual repeatability as well as significant sex and population differences in volatile profiles. The persistence of population differences in a common environment suggests that preen oil chemistry likely has a genetic basis and may thus evolve rapidly in response to environmental change. These finding suggest that songbird preen oil odors have the potential to function as chemosignals associated with mate recognition or reproductive isolation.

Figure 1

(A) A TIC profile obtained from male UCSD junco preen oil, (B) a corresponding postrun single ion current (SIC) profile of methyl ketones (m/z 58) from the time range 25–50 min (1: 2-dodecanone, 2: 2-tridecanone, 3: 2-tetradecanone, 4: 2-pentadecanone, 5: 2-hexadecanone, 5: 2-heptadecanone), and (C) the mass spectrum of 2-pentadecanone from the SIC profile (B).

Figure 2

Principal component 1 and principal component 3 scores derived from relative proportions of volatile compounds. (See Table 2 for principal component loadings.)