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. 2012 Jun;29(6):507-12.
doi: 10.1007/s10815-012-9751-9. Epub 2012 Apr 4.

In-vitro maturation of human oocytes: before or after vitrification?

Giovanna Fasano  1 , Isabelle Demeestere, Yvon Englert
Affiliations

In-vitro maturation of human oocytes: before or after vitrification?

Giovanna Fasano et al. J Assist Reprod Genet. 2012 Jun.

Abstract

Purpose: This study aims to determine if in-vitro maturation (IVM) of human immature oocytes should be performed before or after vitrification.

Methods: A total of 184 immature oocytes were randomly divided into two different groups: 100 were vitrified at metaphase II (MII) stage 24 h-48 h after IVM (group 1) and 84 were immediately vitrified at germinal vesicle (GV) or metaphase I (MI) stages and in vitro matured after warming (group 2).

Results: Survival rate after warming was similar in both groups (86.9% versus 84.5%). However, oocyte maturation rate per collected oocyte was significantly higher for oocytes matured before vitrification (group 1, 46%) than for oocytes vitrified before IVM (group 2, 23.8%) (p < 0.01). Consequently, the number of MII oocytes inseminated per oocyte collected was significantly higher for group 1 (40%) than for group 2 (23.8%) (p < 0.05).

Conclusion: IVM procedure is more efficient when it is performed before oocyte vitrification.

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Figures

Fig. 1
Fig. 1
Experimental design used to test the efficiency of vitrification before or after in vitro maturation (IVM) of oocytes collected at germinal vesicle (GV) and metaphase I (MI) stages
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