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Review
. 2012 Jun;23(6):1003-10.
doi: 10.1007/s13361-012-0377-z. Epub 2012 Apr 3.

Hydrogen exchange mass spectrometry: are we out of the quicksand?

Roxana E Iacob  1 , John R Engen
Affiliations
Review

Hydrogen exchange mass spectrometry: are we out of the quicksand?

Roxana E Iacob et al. J Am Soc Mass Spectrom. 2012 Jun.

Abstract

Although the use of hydrogen exchange (HX) mass spectrometry (MS) to study proteins and protein conformation is now over 20 years old, the perception lingers that it still has "issues." Is this method, in fact, still in the quicksand with many remaining obstacles to overcome? We do not think so. This critical insight addresses the "issues" and explores several broad questions including, have the limitations of HX MS been surmounted and has HX MS achieved "indispensable" status in the pantheon of protein structural analysis tools.

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Figure 1
Figure 1
Questions to ponder about modern hydrogen exchange mass spectrometry.
See this image and copyright information in PMC

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