Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2012 Jun;23(6):1003-10.
doi: 10.1007/s13361-012-0377-z. Epub 2012 Apr 3.

Hydrogen exchange mass spectrometry: are we out of the quicksand?

Roxana E Iacob  1 , John R Engen
Affiliations
Review

Hydrogen exchange mass spectrometry: are we out of the quicksand?

Roxana E Iacob et al. J Am Soc Mass Spectrom. 2012 Jun.

Abstract

Although the use of hydrogen exchange (HX) mass spectrometry (MS) to study proteins and protein conformation is now over 20 years old, the perception lingers that it still has "issues." Is this method, in fact, still in the quicksand with many remaining obstacles to overcome? We do not think so. This critical insight addresses the "issues" and explores several broad questions including, have the limitations of HX MS been surmounted and has HX MS achieved "indispensable" status in the pantheon of protein structural analysis tools.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Questions to ponder about modern hydrogen exchange mass spectrometry.
See this image and copyright information in PMC

References

    1. Katta V, Chait BT. Conformational changes in proteins probed by hydrogen-exchange electrospray-ionization mass spectrometry. Rapid Commun Mass Spectrom. 1991;5:214–217. - PubMed
    1. Anfinsen CB, Redfield RR, Choate WL, Page J, Carroll WR. Studies on the gross structure, cross-linkages, and terminal sequences in ribonuclease. J Biol Chem. 1954;207:201–210. - PubMed
    1. Anfinsen CB. Principles that govern the folding of protein chains. Science. 1973;181:223–230. - PubMed
    1. Zhang Z, Smith DL. Determination of amide hydrogen exchange by mass spectrometry: a new tool for protein structure elucidation. Protein Sci. 1993;2:522–531. - PMC - PubMed
    1. Miranker A, Robinson CV, Radford SE, Dobson CM. Investigation of protein folding by mass spectrometry. FASEB J. 1996;10:93–101. - PubMed

Publication types

MeSH terms