Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Oct;18(3):203-5.
doi: 10.1007/BF00318381.

Detection of length heterogeneity in the ribosomal DNA of Pythium ultimum by PCR amplification of the intergenic region

J Buchko  1 , G R Klassen
Affiliations

Detection of length heterogeneity in the ribosomal DNA of Pythium ultimum by PCR amplification of the intergenic region

J Buchko et al. Curr Genet. 1990 Oct.

Abstract

About one-half of the ribosomal repeat unit of two isolates of Pythium ultimum was amplified by means of the polymerase chain reaction using one primer pair. The amplified region includes a small part of the large subunit ribosomal RNA gene, about half of the small subunit ribosomal RNA gene, and the entire intergenic region. The intergenic region of both isolates of P. ultimum has length heterogeneity due to the presence of subrepeat arrays (Klassen and Buchko 1990). PCR amplification of the heterogeneous target DNA resulted in sets of fragments which accurately reflect the heterogeneity in the target DNA, although there is a preferential amplification of the smaller targets. PCR product sizes ranged from 4.6 to 5.8 kb.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1987 Jan;84(2):610-4 - PubMed
    1. Cell. 1984 Sep;38(2):349-51 - PubMed
    1. Nucleic Acids Res. 1988;16 Suppl:r175-269 - PubMed
    1. Nucleic Acids Res. 1988 Dec 9;16(23):10953-71 - PubMed
    1. Science. 1988 Jan 29;239(4839):487-91 - PubMed

Publication types

LinkOut - more resources