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. 1990 Oct;18(3):265-7.
doi: 10.1007/BF00318391.

Flow cytometry as a tool to discriminate respiratory-competent and respiratory-deficient yeast cells

P Skowronek  1 , G Krummeck, O Haferkamp, G Rödel
Affiliations

Flow cytometry as a tool to discriminate respiratory-competent and respiratory-deficient yeast cells

P Skowronek et al. Curr Genet. 1990 Oct.

Abstract

The cationic lipophilic dye Rhodamine 123 (Rh123) is selectively enriched in mitochondria in a membrane potential-dependent manner. Application of drugs which interfere with the electron flow of the respiratory chain lead to a severe reduction of mitochondrial dye uptake. In this communication we show that the same effect is observed after Rh123-staining of respiratory-deficient yeast mutants. Based on this observation we used flow cytometry to discriminate respiratory-competent and respiratory-deficient yeast cells. Combined with a cell sorter we were able to selectively enrich respiring and non-respiring yeast cells, respectively, from a mixture of cells.

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