Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Jun;50(6):2179-82.
doi: 10.1128/JCM.00503-12. Epub 2012 Apr 4.

Detection of OmpK36 porin loss in Klebsiella spp. by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Jia Chang Cai, Yan Yan Hu, Rong Zhang, Hong Wei Zhou, Gong-Xiang Chen

Detection of OmpK36 porin loss in Klebsiella spp. by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Jia Chang Cai et al. J Clin Microbiol. 2012 Jun.
No abstract available

PubMed Disclaimer

Figures

Fig 1
Fig 1
SDS-PAGE of OMPs from carbapenem-susceptible K. pneumoniae strains (S1 to S5 and ATCC 13883) and carbapenem-resistant Klebsiella strains (K. pneumoniae Z2110, Z2554, K1, K10, Z4, and Z5 and K. oxytoca ZC101). Strains were grown in Mueller-Hinton broth (Oxoid, England) overnight at 37°C with shaking, and outer membrane proteins (OMPs) were extracted as described by Hernandez-Alles et al. (6). OMPs were separated in 11.6% acrylamide-0.4% bisacrylamide-0.1% SDS gels. The gel was run at a constant current of 20 mA and was visualized by staining with Coomassie brilliant blue.
Fig 2
Fig 2
(a) Mass spectra of OMPs from five clinical isolates of carbapenem-susceptible K. pneumoniae. x axis, mass per charge in daltons (m/z, Da); y axis, absolute intensity of signal. No signal was detected in the mass range between 40,000 Da and 80,000 Da, and these regions were not displayed. The mass spectrum was analyzed using FlexAnalysis 3.3 software. The software performed smoothing and baseline subtraction and created a list of the most significant peaks of the spectrum. The arrows with solid lines indicate OmpK35 and OmpK36. (b and c) Mass spectra of OMPs from carbapenem-resistant K. pneumoniae Z2110, Z2554, K1, and K10 (b) and carbapenem-resistant K. pneumoniae Z4 and Z5 and K. oxytoca ZC101 (c). The arrows with dotted lines indicate the loss of 19,000- and 38,000-m/z peaks representing OmpK36.
Fig 2
Fig 2
(a) Mass spectra of OMPs from five clinical isolates of carbapenem-susceptible K. pneumoniae. x axis, mass per charge in daltons (m/z, Da); y axis, absolute intensity of signal. No signal was detected in the mass range between 40,000 Da and 80,000 Da, and these regions were not displayed. The mass spectrum was analyzed using FlexAnalysis 3.3 software. The software performed smoothing and baseline subtraction and created a list of the most significant peaks of the spectrum. The arrows with solid lines indicate OmpK35 and OmpK36. (b and c) Mass spectra of OMPs from carbapenem-resistant K. pneumoniae Z2110, Z2554, K1, and K10 (b) and carbapenem-resistant K. pneumoniae Z4 and Z5 and K. oxytoca ZC101 (c). The arrows with dotted lines indicate the loss of 19,000- and 38,000-m/z peaks representing OmpK36.
See this image and copyright information in PMC

References

    1. Bizzini A, Greub G. 2010. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification. Clin. Microbiol. Infect. 16:1614–1619 - PubMed
    1. Burckhardt I, Zimmermann S. 2011. Using matrix-assisted laser desorption ionization-time of flight mass spectrometry to detect carbapenem resistance within 1 to 2.5 hours. J. Clin. Microbiol. 49:3321–3324 - PMC - PubMed
    1. Cai JC, Zhou HW, Zhang R, Chen GX. 2008. Emergence of Serratia marcescens, Klebsiella pneumoniae, and Escherichia coli isolates possessing the plasmid-mediated carbapenem-hydrolyzing beta-lactamase KPC-2 in intensive care units of a Chinese hospital. Antimicrob. Agents Chemother. 52:2014–2018 - PMC - PubMed
    1. Chen LR, Zhou HW, Cai JC, Zhang R, Chen GX. 2009. Combination of IMP-4 metallo-beta-lactamase production and porin deficiency causes carbapenem resistance in a Klebsiella oxytoca clinical isolate. Diagn. Microbiol. Infect. Dis. 65:163–167 - PubMed
    1. De Carolis E, et al. 27 July 2011. Species identification of Aspergillus, Fusarium and Mucorales with direct surface analysis by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin. Microbiol. Infect. [Epub ahead of print.] doi: - DOI - PubMed

MeSH terms

LinkOut - more resources