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Review
. 2012:2012:597439.
doi: 10.1155/2012/597439. Epub 2012 Feb 2.

Human blood-vessel-derived stem cells for tissue repair and regeneration

Chien-Wen Chen  1 , Mirko Corselli, Bruno Péault, Johnny Huard
Affiliations
Review

Human blood-vessel-derived stem cells for tissue repair and regeneration

Chien-Wen Chen et al. J Biomed Biotechnol. 2012.

Abstract

Multipotent stem/progenitor cells with similar developmental potentials have been independently identified from diverse human tissue/organ cultures. The increasing recognition of the vascular/perivascular origin of mesenchymal precursors suggested blood vessels being a systemic source of adult stem/progenitor cells. Our group and other laboratories recently isolated multiple stem/progenitor cell subsets from blood vessels of adult human tissues. Each of the three structural layers of blood vessels: intima, media, and adventitia has been found to include at least one precursor population, that is, myogenic endothelial cells (MECs), pericytes, and adventitial cells (ACs), respectively. MECs and pericytes efficiently regenerate myofibers in injured and dystrophic skeletal muscles as well as improve cardiac function after myocardial infarction. The applications of ACs in vascular remodeling and angiogenesis/vasculogenesis have been examined. Our recent finding that MECs and pericytes can be purified from cryogenically banked human primary muscle cell culture further indicates their potential applications in personalized regenerative medicine.

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Figures

Figure 1
Figure 1
Distinct morphology of the three purified subpopulations of human blood-vessel-derived stem cells (hBVSCs) in culture: myogenic endothelial cell (MEC), pericyte, and adventitial cell (AC) (100x).
Figure 2
Figure 2
Schematic depiction of hBVSCs at the origin of mesenchymal stem/stromal cells (MSCs). (A) hBVSCs, including myogenic endothelial cells (MECs, red), pericytes (green), and adventitial cells (AC, blue), are dissociated from fresh muscle biopsy and separated from endothelial cells (yellow) and other cell types. (B) Dissociated cells are purified to homogeneity by fluorescence-activated cell sorting (FACS) and newly sorted MECs, pericytes, and ACs already exhibit multilineage developmental potentials. (C) FACS-purified pericytes, ACs, and possibly MECs give rise to authentic MSCs in long-term culture. (D) Nevertheless, whether native hBVSCs serve as a source of MSCs in situ and participate in tissue repair and regeneration remains an open question.
See this image and copyright information in PMC

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