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. 2012 Mar;17(3):030502.
doi: 10.1117/1.JBO.17.3.030502.

Optical coherence photoacoustic microscopy: accomplishing optical coherence tomography and photoacoustic microscopy with a single light source

Xiangyang Zhang, Hao F Zhang, Shuliang Jiao

Optical coherence photoacoustic microscopy: accomplishing optical coherence tomography and photoacoustic microscopy with a single light source

Xiangyang Zhang et al. J Biomed Opt. 2012 Mar.

Abstract

We developed optical coherence photoacoustic microscopy (OC-PAM) to demonstrate that the functions of optical coherence tomography (OCT) and photoacoustic microscopy (PAM) can be achieved simultaneously by using a single illuminating light source. We used a pulsed broadband laser centered at 580 nm and detected the absorbed photons through photoacoustic detection and the back-scattered photons with an interferometer. In OC-PAM, each laser pulse generates both one OCT A-line and one PAM A-line simultaneously; as a result, the two imaging modalities are intrinsically co-registered in the lateral directions. In vivo images of the mouse ear were acquired to demonstrate the capabilities of OC-PAM.

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Figures

Fig. 1
Fig. 1
Schematic of the experimental system of a free-space OC-PAM. L1 to L4: lens; BS: beam splitter; SMF: single mode fiber; PD: photodiode; UT: ultrasonic transducer; M1, M2: mirror.
Fig. 2
Fig. 2
Performance of the OCT mode. (a) The measured spectrum of the light source. (b) The calculated PSF of the OCT subsystem.
Fig. 3
Fig. 3
OCT and PAM B-scan images acquired simultaneously in vivo. (a) OCT B-scan image; (b) PAM B-scan image, and (c) maximum-amplitude-projection (MAP) of the 3D PAM dataset. The vertical lines marked the corresponding locations of the recognized blood vessels. The horizontal line in the MAP image marks the location of the displayed B-scans; bar: 100 μm.
Fig. 4
Fig. 4
Fused OCT and PAM images shown in Fig. 3; bar: 100 μm.
See this image and copyright information in PMC

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