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. 2012:2012:396316.
doi: 10.1155/2012/396316. Epub 2012 Mar 15.

Evaluation of osteoconductive and osteogenic potential of a dentin-based bone substitute using a calvarial defect model

Ibrahim Hussain  1 , Keyvan Moharamzadeh, Ian M Brook, Patrício José de Oliveira Neto, Luiz A Salata
Affiliations

Evaluation of osteoconductive and osteogenic potential of a dentin-based bone substitute using a calvarial defect model

Ibrahim Hussain et al. Int J Dent. 2012.

Abstract

The aim of this study was to assess the osteoconductive and osteogenic properties of processed bovine dentin using a robust rabbit calvarial defect model. In total, 16 New Zealand White rabbits were operated to create three circular defects in the calvaria. One defect was left unfilled, one filled with collected autogenous bone, and the third defect was filled with the dentin-based bone substitute. Following surgery and after a healing period of either 1 or 6 weeks, a CT scan was obtained. Following sacrificing, the tissues were processed for histological examination. The CT data showed the density in the area grafted with the dentin-based material was higher than the surrounding bone and the areas grafted with autologous bone after 1 week and 6 weeks of healing. The area left unfilled remained an empty defect after 1 week and 6 weeks. Histological examination of the defects filled with the dentin product after 6 weeks showed soft tissue encapsulation around the dentin particles. It can be concluded that the rabbit calvarial model used in this study is a robust model for the assessment of bone materials. Bovine dentin is a biostable material; however, it may not be suitable for repairing large 4-wall defects.

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Figures

Figure 1
Figure 1
Surgical defects on rabbit calvarium filled with test materials. One defect was left unfilled (upper right), one filled with collected particulate autogenous bone (upper left), and the third defect was filled with the new bovine dentin bone substitute (lower right).
Figure 2
Figure 2
Post-op CT scan of (a) unfilled area immediately after surgery; (b) unfilled area 1 week post operatively; (c) dentin-filled defect (left arrow) and the autogenous bone-filled defect (right arrow) immediately after surgery; (d) dentin-filled defect (left arrow) and the autogenous bone-filled defect (right arrow) 1 week postoperatively.
Figure 3
Figure 3
Post-op CT scan of (a) unfilled area immediately after surgery; (b) unfilled area 6 weeks postoperatively; (c) dentin-filled defect (right arrow) and the autogenous bone-filled defect (left arrow) immediately after surgery; (d) dentin-filled defect (right arrow) and the autogenous bone-filled defect (left arrow) 6 weeks postoperatively.
Figure 4
Figure 4
Mallory's trichrome-stained histological sections of defect areas filled with (a) processed dentin product, 1 week post-op, original magnification ×10; (b) processed dentin product, 6 weeks post-op, original magnification ×10; (c) autogenous bone, 1 week post-op original magnification ×20; (d) autogenous bone, 6 weeks post-op, original magnification ×20.
See this image and copyright information in PMC

References

    1. Fugazzotto PA, De Paoli S, Benfenati SP. The use of allogenic freeze-dried dentin in the repair of periodontal osseous defects in humans. Quintessence International. 1986;17(8):461–477. - PubMed
    1. Gomes MF, Da Silva Dos Anjos MJ, De Nogueira TO, Catanzaro-Guimarães SA. Autogenous demineralized dentin matrix for tissue engineering applications: radiographic and histomorphometric studies. International Journal of Oral and Maxillofacial Implants. 2002;17(4):488–497. - PubMed
    1. Gomes MF, Da Silva Dos Anjos MJ, De Oliveira Nogueira T, Catanzaro Guimarães SA. Histologic evaluation of the osteoinductive property of autogenous demineralized dentin matrix on surgical bone defects in rabbit skulls using human amniotic membrane for guided bone regeneration. International Journal of Oral and Maxillofacial Implants. 2001;16(4):563–571. - PubMed
    1. Su-Gwan K, Hak-Kyun K, Sung-Chul L. Combined implantation of particulate dentine, plaster of Paris, and a bone xenograft (Bio-Oss®) for bone regeneration in rats. Journal of Cranio-Maxillofacial Surgery. 2001;29(5):282–288. - PubMed
    1. Moharamzadeh K, Freeman C, Blackwood K. Processed bovine dentine as a bone substitute. British Journal of Oral and Maxillofacial Surgery. 2008;46(2):110–113. - PubMed