Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 May;50(5):393-5.
doi: 10.1093/chromsci/bmr004.

Simple and low-cost high-performance liquid chromatographic method for determination of D- and L-amino acids

Miki Tojo  1 , Masahito Murakami, Yoko Nagata
Affiliations

Simple and low-cost high-performance liquid chromatographic method for determination of D- and L-amino acids

Miki Tojo et al. J Chromatogr Sci. 2012 May.

Abstract

In this article, a simple and low-cost method for the analysis of amino acid enantiomers by using high-performance liquid chromatography (HPLC) is described. In this method, the amino acids are modified to diastereomers in order to be separated into enantiomers on a usual C(18) reversed-phase column. Methanol instead of acetonitrile is used as an elution solvent; the results of HPLC with methanol elution are comparable with those of HPLC with acetonitrile elution. Sub-nanomolar sensitivity is attained by measuring the absorbance at 340 nm in analysis of 15 amino-acid enantiomers.

PubMed Disclaimer

Publication types

LinkOut - more resources