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. 1990;76(8):648-52.
doi: 10.1007/BF00931081.

An improved ELISA for the detection of antibodies against Babesia bovis using either a native or a recombinant B. bovis antigen

R Böse  1 , R H Jacobson, K R Gale, D J Waltisbuhl, I G Wright
Affiliations

An improved ELISA for the detection of antibodies against Babesia bovis using either a native or a recombinant B. bovis antigen

R Böse et al. Parasitol Res. 1990.

Abstract

Two new enzyme-linked immunosorbent assays (ELISA) for the diagnosis of Babesia bovis in cattle are described. The ELISA using a native antigen is more sensitive and less laborious than the assays described previously, because it does not require adsorption of sera with bovine erythrocytes. The second ELISA, using a recombinant B. bovis antigen expressed in Escherichia coli, was both sensitive and specific. It is suitable to replace the native antigen, thus avoiding large batch-to-batch variations in antigen preparations and the need to sacrifice experimental cattle.

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