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. 2012 Apr;22(4):530-4.
doi: 10.4014/jmb.1107.07064.

Development of real-time PCR for the detection of Clostridium perfringens in meats and vegetables

Jung-Whan Chon  1 , Jong-Seok Park, Ji-Yeon Hyeon, Chankyu Park, Kwang-Young Song, Kwang-Won Hong, In-Gyun Hwang, Hyo-Sun Kwak, Kun-Ho Seo
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Free article

Development of real-time PCR for the detection of Clostridium perfringens in meats and vegetables

Jung-Whan Chon et al. J Microbiol Biotechnol. 2012 Apr.
Free article

Abstract

A real-time PCR assay was developed and validated inhouse specifically for the detection of Clostridium perfringens (Cl. perfringens) in meats and vegetables by comparing with the culture method. The detection limit of the real-time PCR assay in phosphate-buffered saline was 10² CFU/ml. When the two methods were compared in food samples inoculated with Cl. perfringens, the culture method detected 52 positives, whereas real-time PCR detected 51 positives out of 160 samples. The difference was without statistical significance (p>0.05). Real-time PCR assay is an option for quality assurance laboratories to perform standard diagnostic tests, considering its detection ability and time-saving efficiency.

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