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. 1990 Nov;86(1):14-6.
doi: 10.1007/BF00205165.

R-banding and nonisotopic in situ hybridization: precise localization of the human type II collagen gene (COL2A1)

E Takahashi  1 , T Hori, P O'Connell, M Leppert, R White
Affiliations

R-banding and nonisotopic in situ hybridization: precise localization of the human type II collagen gene (COL2A1)

E Takahashi et al. Hum Genet. 1990 Nov.

Abstract

A new mapping system, based on nonisotopic in situ hybridization combined with fluorescent staining of replicated prometaphase R-bands, is described. Replication of the bands is achieved by treatment of thymidine-synchronized cells with bromodeoxyuridine. The human COL2A1 gene was mapped to band 12q13.11-q13.12 in this manner, to illustrate the potential of the technique for improving the precision of chromosomal mapping and physical ordering of genes.

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References

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