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. 2012 Apr 27:6:35.
doi: 10.1186/1752-153X-6-35.

Evaluation of Biologically Active Compounds from Calendula officinalis Flowers using Spectrophotometry

Monica Butnariu  1 , Cristina Zepa Coradini
Affiliations

Evaluation of Biologically Active Compounds from Calendula officinalis Flowers using Spectrophotometry

Monica Butnariu et al. Chem Cent J. .

Abstract

Background: This study aimed to quantify the active biological compounds in C. officinalis flowers. Based on the active principles and biological properties of marigolds flowers reported in the literature, we sought to obtain and characterize the molecular composition of extracts prepared using different solvents. The antioxidant capacities of extracts were assessed by using spectrophotometry to measure both absorbance of the colorimetric free radical scavenger 2,2-diphenyl-1-picrylhydrazyl (DPPH) as well as the total antioxidant potential, using the ferric reducing power (FRAP) assay.

Results: Spectrophotometric assays in the ultraviolet-visible (UV-VIS) region enabled identification and characterization of the full range of phenolic and flavonoids acids, and high-performance liquid chromatography (HPLC) was used to identify and quantify phenolic compounds (depending on the method of extraction). Methanol ensured more efficient extraction of flavonoids than the other solvents tested.Antioxidant activity in methanolic extracts was correlated with the polyphenol content.

Conclusions: The UV-VIS spectra of assimilator pigments (e.g. chlorophylls), polyphenols and flavonoids extracted from the C. officinalis flowers consisted in quantitative evaluation of compounds which absorb to wavelengths broader than 360 nm.

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Figures

Figure 1
Figure 1
Contents of compounds with antioxidant potential from C. officinalis extracts.
Figure 2
Figure 2
The absorption spectrum of 80% methanolic flower extracts of C. officinalis.
Figure 3
Figure 3
Absorption spectra of C. officinalis flower in methanol 80%, ethanol 96%, isopropanol 99% and ethanol 60%.
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